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csf1 cytokine  (PeproTech)


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    Structured Review

    PeproTech csf1 cytokine
    Csf1 Cytokine, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/csf1+cytokine/recombinant+murine+gm+csf/pmc11893137-475-31-32
    Average 90 stars, based on 1 article reviews
    csf1 cytokine - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Red Blood Cell Lysis:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Cell Culture:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Incubation:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Enzyme-linked Immunosorbent Assay:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Infection:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Flow Cytometry:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Two Tailed Test:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Control:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Virus:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Cell Function Assay:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Functional Assay:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Isolation:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Staining:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    MANN-WHITNEY:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    In Vitro:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Immunohistochemistry:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Blocking Assay:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    RNA Sequencing:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Quantitative Proteomics:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Expressing:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Gene Expression:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Inhibition:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Comparison:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Multiplex Assay:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    BrdU Incorporation Assay:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Derivative Assay:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Co-Culture Assay:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Transwell Assay:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Ab Array:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Knockdown:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Western Blot:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Microarray:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.

    Quantitative RT-PCR:

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300-25-10), 80 ng/mL IL10 (PeproTech, 200-10-10) or both were added to 24-well plates and incubated for 3 days.

    Article Title: EBV promotes TCR-T-cell therapy resistance by inducing CD163+M2 macrophage polarization and MMP9 secretion.
    Article Snippet: Alternatively, for differentiation induction with cytokines, 50 ng/mL CSF1 (PeproTech, 300- 25- 10), 80 ng/mL IL10 (PeproTech, 200- 10- 10) or both were added to 24- well plates and incubated for 3 days.

    Article Title: Transfection of Vitamin D3-Induced Tolerogenic Dendritic Cells for the Silencing of Potential Tolerogenic Genes. Identification of CSF1R-CSF1 Signaling as a Glycolytic Regulator
    Article Snippet: To further analyze the role of CD115 signaling during monocyte differentiation, 100 ng/mL of their ligands, CSF1 and IL34 (both from Peprotech), was added independently or in combination to the culture of mDC and tolDC on day 0 and day 4.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma.
    Article Snippet: OT-1 cells were activated with 1 nM OVA-I peptide (SIINFEKL) (Sigma-Aldrich) in the presence of 100U/ml interleukin-2 (IL-2) (PeproTech) in vitro for 3 days.

    Article Title: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
    Article Snippet: After red blood cell lysis, bone marrow cells were seeded at a density of 10 7 cells/100 × 15-mm Petri dish and cultured at 37 °C in DMEM containing 20 ng/ml CSF1 (PeproTech).

    Article Title: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis
    Article Snippet: BM cells were cultured in RPMI-1640 (Gibco) with 10 % FBS (Bio-Sera), 10 units/ml penicillin (Gibco), 0.1 mg/ml streptomycin (Gibco), 2.5 μg/ml amphotericin B (Gibco) and 50 μM 2-mercaptoethanol (Gibco), together with 20 ng/ml CSF2 (Peprotech) or 100 ng/ml CSF1 (Peprotech).

    Article Title: Macrophage proliferation machinery leads to PDAC progression, but susceptibility to innate immunotherapy
    Article Snippet: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs.

    Article Title: HIV-1 Tat Upregulates TREM1 Expression in Human Microglia
    Article Snippet: In brief, 6 × 10 6 PBMCs/ml were incubated in MMG media (RPMI 1640 GlutaMAX supplemented with 100 μg/ml streptomycin; 100 U/ml penicillin [all Gibco]; 10 ng/ml each of CSF1, CSF2, and nerve growth factor β; and 100 ng/ml CCL2 and IL-34 [all PeproTech]) for 4 h, after which nonadherent cells were removed by aspiration.



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    Image Search Results


    Interleukin-34 (IL-34) and colony-stimulating factor 1 (CSF1) equally differentiate blood monocytes into macrophages. Flow cytometry analysis of (a) CD14 and (b) CD163 on undifferentiated monocytes as well as CSF1 and IL-34 differentiated macrophages. The mean fluorescence intensity (MFI) of CD14 is not significantly different between all three cell types, while CD163 expression is increased significantly upon differentiation of macrophages in the presence of both IL-34 and CSF1. In all cases, ≥ 95% of differentiated macrophages were positive for CD14 and CD163. Shown is the average ± standard error of 4–9 independent experiments, * indicates p < 0.05 by one-way ANOVA with Dunnett’s multiple comparison

    Journal: Molecular oral microbiology

    Article Title: Interleukin-34 permits Porphyromonas gingivalis survival and NF- κ B p65 inhibition in macrophages

    doi: 10.1111/omi.12366

    Figure Lengend Snippet: Interleukin-34 (IL-34) and colony-stimulating factor 1 (CSF1) equally differentiate blood monocytes into macrophages. Flow cytometry analysis of (a) CD14 and (b) CD163 on undifferentiated monocytes as well as CSF1 and IL-34 differentiated macrophages. The mean fluorescence intensity (MFI) of CD14 is not significantly different between all three cell types, while CD163 expression is increased significantly upon differentiation of macrophages in the presence of both IL-34 and CSF1. In all cases, ≥ 95% of differentiated macrophages were positive for CD14 and CD163. Shown is the average ± standard error of 4–9 independent experiments, * indicates p < 0.05 by one-way ANOVA with Dunnett’s multiple comparison

    Article Snippet: Macrophages were generated from blood monocytes in six-well culture plates in culture medium (Roswell Park Memorial Institute (RPMI)) 1640 medium [Corning] containing 10% Fetal Bovine Serum (FBS) (Caisson Labs), 100 IU/ml penicillin, 100 μ g/ml streptomycin, and 0.25 μ g/ml amphotericin) for 6 days supplemented with 50 ng/ml CSF1 or 50 ng/ml IL-34 (GenScript; Foucher et al., 2013 ).

    Techniques: Flow Cytometry, Fluorescence, Expressing, Comparison

    IL-34 macrophages are less efficient at killing internalized Porphyromonas gingivalis. (a) CSF1 and IL-34 macrophages are equally efficient at internalizing P. gingivalis. Shown are the average (± standard error) internalized bacteria per macrophage after 30 min co-incubation (MOI 10:1) of four independent experiments with bacteria quantified within a minimum of 100 macrophages per experiment. (b) Percent survival of internalized P. gingivalis within macrophages for 60 or 120 min post-uptake using an antibiotic protection assay. Shown is the mean (± standard error) of 3–4 independent experiments, p-value calculated with (a) an unpaired t-test and (b) a two-way ANOVA followed by Šídák’s multiple comparison test of survival of P. gingivalis within IL-34 and CSF1-differentiated macrophages at each timepoint

    Journal: Molecular oral microbiology

    Article Title: Interleukin-34 permits Porphyromonas gingivalis survival and NF- κ B p65 inhibition in macrophages

    doi: 10.1111/omi.12366

    Figure Lengend Snippet: IL-34 macrophages are less efficient at killing internalized Porphyromonas gingivalis. (a) CSF1 and IL-34 macrophages are equally efficient at internalizing P. gingivalis. Shown are the average (± standard error) internalized bacteria per macrophage after 30 min co-incubation (MOI 10:1) of four independent experiments with bacteria quantified within a minimum of 100 macrophages per experiment. (b) Percent survival of internalized P. gingivalis within macrophages for 60 or 120 min post-uptake using an antibiotic protection assay. Shown is the mean (± standard error) of 3–4 independent experiments, p-value calculated with (a) an unpaired t-test and (b) a two-way ANOVA followed by Šídák’s multiple comparison test of survival of P. gingivalis within IL-34 and CSF1-differentiated macrophages at each timepoint

    Article Snippet: Macrophages were generated from blood monocytes in six-well culture plates in culture medium (Roswell Park Memorial Institute (RPMI)) 1640 medium [Corning] containing 10% Fetal Bovine Serum (FBS) (Caisson Labs), 100 IU/ml penicillin, 100 μ g/ml streptomycin, and 0.25 μ g/ml amphotericin) for 6 days supplemented with 50 ng/ml CSF1 or 50 ng/ml IL-34 (GenScript; Foucher et al., 2013 ).

    Techniques: Bacteria, Incubation, Comparison

    IL-34 downregulates intracellular reactive oxygen species (ROS) production from macrophages. Luminol-based ROS detection was used to quantify the ROS response by CSF1- and IL-34-differentiated macrophages during the activation with phorbol myristate acetate (100 nm). (a) Representative panel for the time course of luminol luminescence detection, shown in relative luminescence units (RLU). (b) Mean peak RLU (with standard error) was detected over three independent experiments. * p < 0.01 (paired t-test)

    Journal: Molecular oral microbiology

    Article Title: Interleukin-34 permits Porphyromonas gingivalis survival and NF- κ B p65 inhibition in macrophages

    doi: 10.1111/omi.12366

    Figure Lengend Snippet: IL-34 downregulates intracellular reactive oxygen species (ROS) production from macrophages. Luminol-based ROS detection was used to quantify the ROS response by CSF1- and IL-34-differentiated macrophages during the activation with phorbol myristate acetate (100 nm). (a) Representative panel for the time course of luminol luminescence detection, shown in relative luminescence units (RLU). (b) Mean peak RLU (with standard error) was detected over three independent experiments. * p < 0.01 (paired t-test)

    Article Snippet: Macrophages were generated from blood monocytes in six-well culture plates in culture medium (Roswell Park Memorial Institute (RPMI)) 1640 medium [Corning] containing 10% Fetal Bovine Serum (FBS) (Caisson Labs), 100 IU/ml penicillin, 100 μ g/ml streptomycin, and 0.25 μ g/ml amphotericin) for 6 days supplemented with 50 ng/ml CSF1 or 50 ng/ml IL-34 (GenScript; Foucher et al., 2013 ).

    Techniques: Activation Assay

    Comparison of the surface expression of markers with known P. gingivalis interactions on IL-34 and CSF1 macrophages. The majority of markers with known interactions with P. gingivalis are not significantly different between CSF1- and IL-34-differentiated macrophages, with the exception of dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin (DC-SIGN). Macrophages matured in the presence of either CSF1 or IL-34 were labeled with CD14 and one or more of C5aR, CD11b, TLR 2, TLR 4, or DC-SIGN and analyzed by flow cytometry. The cells were gated for positive CD14 (≥ 90% of differentiated cells), and the MFI of the additional markers was determined. Representative histograms are shown, along with the mean (± standard error) of at least four independent experiments per marker tested. p-values were determined by paired (IL-34 vs. CSF1) t-tests.

    Journal: Molecular oral microbiology

    Article Title: Interleukin-34 permits Porphyromonas gingivalis survival and NF- κ B p65 inhibition in macrophages

    doi: 10.1111/omi.12366

    Figure Lengend Snippet: Comparison of the surface expression of markers with known P. gingivalis interactions on IL-34 and CSF1 macrophages. The majority of markers with known interactions with P. gingivalis are not significantly different between CSF1- and IL-34-differentiated macrophages, with the exception of dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin (DC-SIGN). Macrophages matured in the presence of either CSF1 or IL-34 were labeled with CD14 and one or more of C5aR, CD11b, TLR 2, TLR 4, or DC-SIGN and analyzed by flow cytometry. The cells were gated for positive CD14 (≥ 90% of differentiated cells), and the MFI of the additional markers was determined. Representative histograms are shown, along with the mean (± standard error) of at least four independent experiments per marker tested. p-values were determined by paired (IL-34 vs. CSF1) t-tests.

    Article Snippet: Macrophages were generated from blood monocytes in six-well culture plates in culture medium (Roswell Park Memorial Institute (RPMI)) 1640 medium [Corning] containing 10% Fetal Bovine Serum (FBS) (Caisson Labs), 100 IU/ml penicillin, 100 μ g/ml streptomycin, and 0.25 μ g/ml amphotericin) for 6 days supplemented with 50 ng/ml CSF1 or 50 ng/ml IL-34 (GenScript; Foucher et al., 2013 ).

    Techniques: Comparison, Expressing, Labeling, Flow Cytometry, Marker

    IL-34-matured macrophages produce less IL-8 than CSF1-matured macrophages. IL-34- or CSF1-differentiated macrophages were co-incubated with P. gingivalis for 24 h, with media samples removed at 2, 6, and 24 h and subsequently analyzed by multiplex ELISA. The analytes that had significant release above control macrophages are shown. Four independent experiments were performed, mean ± standard error is shown. p-values were determined by two-way ANOVA followed by Šídák’s multiple comparison test of IL-34 versus CSF1-differentiated macrophages at each timepoint: those without p-value indicated have a p > 0.05. Significantly less IL-8 was produced by IL-34-differentiated macrophages than by CSF1-differentiated macrophages

    Journal: Molecular oral microbiology

    Article Title: Interleukin-34 permits Porphyromonas gingivalis survival and NF- κ B p65 inhibition in macrophages

    doi: 10.1111/omi.12366

    Figure Lengend Snippet: IL-34-matured macrophages produce less IL-8 than CSF1-matured macrophages. IL-34- or CSF1-differentiated macrophages were co-incubated with P. gingivalis for 24 h, with media samples removed at 2, 6, and 24 h and subsequently analyzed by multiplex ELISA. The analytes that had significant release above control macrophages are shown. Four independent experiments were performed, mean ± standard error is shown. p-values were determined by two-way ANOVA followed by Šídák’s multiple comparison test of IL-34 versus CSF1-differentiated macrophages at each timepoint: those without p-value indicated have a p > 0.05. Significantly less IL-8 was produced by IL-34-differentiated macrophages than by CSF1-differentiated macrophages

    Article Snippet: Macrophages were generated from blood monocytes in six-well culture plates in culture medium (Roswell Park Memorial Institute (RPMI)) 1640 medium [Corning] containing 10% Fetal Bovine Serum (FBS) (Caisson Labs), 100 IU/ml penicillin, 100 μ g/ml streptomycin, and 0.25 μ g/ml amphotericin) for 6 days supplemented with 50 ng/ml CSF1 or 50 ng/ml IL-34 (GenScript; Foucher et al., 2013 ).

    Techniques: Incubation, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Control, Comparison, Produced

    Dephosphorylation of NF-κB p65 is associated with lower levels of IL-8 in IL-34-differentiated macrophages. IL-34- or CSF1-differentiated macrophages were co-incubated with P. gingivalis for 2 h Total cell lysates were assessed by western blot using phospho-NF-κB p65 (Ser-536) or total NF-κB p65 antibodies. Anti-mouse actin was used as an internal control. Data shown in Panel B are representative of three independent experiments with similar results; Panel A shows the resulting densitometry analysis of the ratio between phosphorylated and total NF-κB. Statistical analysis (one-way ANOVA with pairwise comparison) indicates a significant difference in the phosphorylated NF-κB between IL-34- and CSF1-differentiated macrophages incubated with P. gingivalis but not control or LPS-incubated macrophages.

    Journal: Molecular oral microbiology

    Article Title: Interleukin-34 permits Porphyromonas gingivalis survival and NF- κ B p65 inhibition in macrophages

    doi: 10.1111/omi.12366

    Figure Lengend Snippet: Dephosphorylation of NF-κB p65 is associated with lower levels of IL-8 in IL-34-differentiated macrophages. IL-34- or CSF1-differentiated macrophages were co-incubated with P. gingivalis for 2 h Total cell lysates were assessed by western blot using phospho-NF-κB p65 (Ser-536) or total NF-κB p65 antibodies. Anti-mouse actin was used as an internal control. Data shown in Panel B are representative of three independent experiments with similar results; Panel A shows the resulting densitometry analysis of the ratio between phosphorylated and total NF-κB. Statistical analysis (one-way ANOVA with pairwise comparison) indicates a significant difference in the phosphorylated NF-κB between IL-34- and CSF1-differentiated macrophages incubated with P. gingivalis but not control or LPS-incubated macrophages.

    Article Snippet: Macrophages were generated from blood monocytes in six-well culture plates in culture medium (Roswell Park Memorial Institute (RPMI)) 1640 medium [Corning] containing 10% Fetal Bovine Serum (FBS) (Caisson Labs), 100 IU/ml penicillin, 100 μ g/ml streptomycin, and 0.25 μ g/ml amphotericin) for 6 days supplemented with 50 ng/ml CSF1 or 50 ng/ml IL-34 (GenScript; Foucher et al., 2013 ).

    Techniques: De-Phosphorylation Assay, Incubation, Western Blot, Control, Comparison

    IL-34-matured macrophages produce less IL-8 upon NF-kB p65-specific inhibition. Human peripheral blood monocytes were differentiated with CSF1 (50 ng/ml) and IL-34 (50 ng/ml)for 6 days in 24-well tissue culture plates (2 × 105 cells/well). The differentiated macrophages were pre-incubated with NFkB p65 inhibitor (NBP2-29321, 100, μM) and control peptide (NBP2-29334, 100 μM) overnight. Cells were then stimulated with LPS (0.1 μg/ml) for 16 h. The cell supernatants were analyzed for the production of IL-8 by ELISA. Shown is the average ± standard error, **** indicates p < 0.0001 by one-way ANOVA with Dunnett’s multiple comparison test

    Journal: Molecular oral microbiology

    Article Title: Interleukin-34 permits Porphyromonas gingivalis survival and NF- κ B p65 inhibition in macrophages

    doi: 10.1111/omi.12366

    Figure Lengend Snippet: IL-34-matured macrophages produce less IL-8 upon NF-kB p65-specific inhibition. Human peripheral blood monocytes were differentiated with CSF1 (50 ng/ml) and IL-34 (50 ng/ml)for 6 days in 24-well tissue culture plates (2 × 105 cells/well). The differentiated macrophages were pre-incubated with NFkB p65 inhibitor (NBP2-29321, 100, μM) and control peptide (NBP2-29334, 100 μM) overnight. Cells were then stimulated with LPS (0.1 μg/ml) for 16 h. The cell supernatants were analyzed for the production of IL-8 by ELISA. Shown is the average ± standard error, **** indicates p < 0.0001 by one-way ANOVA with Dunnett’s multiple comparison test

    Article Snippet: Macrophages were generated from blood monocytes in six-well culture plates in culture medium (Roswell Park Memorial Institute (RPMI)) 1640 medium [Corning] containing 10% Fetal Bovine Serum (FBS) (Caisson Labs), 100 IU/ml penicillin, 100 μ g/ml streptomycin, and 0.25 μ g/ml amphotericin) for 6 days supplemented with 50 ng/ml CSF1 or 50 ng/ml IL-34 (GenScript; Foucher et al., 2013 ).

    Techniques: Inhibition, Incubation, Control, Enzyme-linked Immunosorbent Assay, Comparison